Faculty Mentor

Enmin Zou

Location

Farrar Hall, Room 133

Session

Session 3

Start Date

20-4-2012 9:30 AM

End Date

20-4-2012 10:30 AM

Description

If crustacean ethoxyresorufin O-deethylase (EROD) activity varies with the molting physiology, then the use of random crustacean EROD activity and whole tissue samples as biomarkers for pollution can be questioned. This study determined if mitochondrial or microsomal EROD activities in the hepatopancreas were affected by the molting physiology in the fiddler crab, Uca pugilator. Intermolt crabs were injected with the exogenous molting hormone 20-hydroxyecdsyone. Results showed a significant increase in mitochondrial EROD activity suggesting that mitochondrial EROD activity is influenced by molting physiology. Only microsomal cell fractions should be used to perform crustacean EROD assays when evaluating aquatic pollution.

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Apr 20th, 9:30 AM Apr 20th, 10:30 AM

Is EROD Activity Affected by the Molting Cycle in the Fiddler Crab, Uca pugilator?

Farrar Hall, Room 133

If crustacean ethoxyresorufin O-deethylase (EROD) activity varies with the molting physiology, then the use of random crustacean EROD activity and whole tissue samples as biomarkers for pollution can be questioned. This study determined if mitochondrial or microsomal EROD activities in the hepatopancreas were affected by the molting physiology in the fiddler crab, Uca pugilator. Intermolt crabs were injected with the exogenous molting hormone 20-hydroxyecdsyone. Results showed a significant increase in mitochondrial EROD activity suggesting that mitochondrial EROD activity is influenced by molting physiology. Only microsomal cell fractions should be used to perform crustacean EROD assays when evaluating aquatic pollution.