Event Title
Characterization of Ubiquitin/Proteasome-Dependent Degradation of Hap4 in the Regulation of Hap2/3/4
Co-investigators
Denise Capps; Mengying Chiang; Tammy Pracheil
Faculty Mentor
Zhengchang Liu
Location
Farrar Hall, Room 133
Session
Session 3
Start Date
20-4-2012 9:30 AM
End Date
20-4-2012 10:30 AM
Description
The Hap2/3/4/5 complex is a heme-activated, CCAATT binding, global transcriptional activator of genes involved in respiration and mitochondrial biogenesis in yeast species Saccharomyces cerevisiae. Hap4 is the regulatory subunit of the complex and its levels determine the activity of the complex. Little is known about the regulation of Hap4 levels or its response to a cell’s functional state. It has been observed that the activity of Hap2-5 complex is reduced in respiratory-deficient cells. We have found a link between Hap4 stability, as mediated through 26S proteasome degradation and dependence on mitochondrial functional state, as playing a regulatory role on downstream targets of the Hap complex.
Characterization of Ubiquitin/Proteasome-Dependent Degradation of Hap4 in the Regulation of Hap2/3/4
Farrar Hall, Room 133
The Hap2/3/4/5 complex is a heme-activated, CCAATT binding, global transcriptional activator of genes involved in respiration and mitochondrial biogenesis in yeast species Saccharomyces cerevisiae. Hap4 is the regulatory subunit of the complex and its levels determine the activity of the complex. Little is known about the regulation of Hap4 levels or its response to a cell’s functional state. It has been observed that the activity of Hap2-5 complex is reduced in respiratory-deficient cells. We have found a link between Hap4 stability, as mediated through 26S proteasome degradation and dependence on mitochondrial functional state, as playing a regulatory role on downstream targets of the Hap complex.