Event Title
Use of Novel Restriction Digestion Patterns to Predict Cluster Assignment of Mycobacteriophage
Co-investigators
Daniel Bonnette
Faculty Mentor
A.M. Findley; C.R. Gissendanner; A.D.M. Wiedemeier
Location
Memorial Gym
Start Date
20-4-2012 2:30 PM
End Date
20-4-2012 3:30 PM
Description
Mycobacteriophages were isolated from soil samples via direct plating or enrichment. Isolates were subjected to spot testing, repetitive purification plating, and an empirical testing protocol that led to the harvesting of high-titer lysates. DNA was isolated from each phage and characterized with a battery of 13 restriction enzymes to gain additional insight into their probable cluster assignment. Our analysis resulted in the following predictions: Hades = F1; JAWS = K; Tarball = A4. Mycobacteriophage sp. JAWS and Hades were finished and annotated using the Consed, Glimmer/GeneMark and Phamerator programs and the predictive power of our approach was confirmed.
Use of Novel Restriction Digestion Patterns to Predict Cluster Assignment of Mycobacteriophage
Memorial Gym
Mycobacteriophages were isolated from soil samples via direct plating or enrichment. Isolates were subjected to spot testing, repetitive purification plating, and an empirical testing protocol that led to the harvesting of high-titer lysates. DNA was isolated from each phage and characterized with a battery of 13 restriction enzymes to gain additional insight into their probable cluster assignment. Our analysis resulted in the following predictions: Hades = F1; JAWS = K; Tarball = A4. Mycobacteriophage sp. JAWS and Hades were finished and annotated using the Consed, Glimmer/GeneMark and Phamerator programs and the predictive power of our approach was confirmed.
Comments
Poster presentation