Event Title
Phytoestrogen Variation Among Organs of Soybeans over the Span of Three Growth Stages
Co-investigators
Sunita Maharjan
Faculty Mentor
Thea M. Edwards
Location
Memorial Gym
Start Date
20-4-2012 2:30 PM
End Date
20-4-2012 3:30 PM
Description
We collected tissue samples from different organs of soybeans at various growth stages to examine how phytoestrogen concentrations vary throughout the plant over time. For phytoestrogen analysis, fresh plant tissues from leaves, roots, shoots, stems, seeds, pods, and flowers were disrupted using a blender or mortar and pestle and extracted for 24 to 48 hours in 55% ethanol. Extracts were filtered, concentrated by evaporation under nitrogen, and reconstituted to a known concentration. Extracts were tested for estrogenicity using a yeast reporter gene system expressing human ESR1 and ESR2. Our results suggest that phytoestrogen concentrations vary among plant organs.
Phytoestrogen Variation Among Organs of Soybeans over the Span of Three Growth Stages
Memorial Gym
We collected tissue samples from different organs of soybeans at various growth stages to examine how phytoestrogen concentrations vary throughout the plant over time. For phytoestrogen analysis, fresh plant tissues from leaves, roots, shoots, stems, seeds, pods, and flowers were disrupted using a blender or mortar and pestle and extracted for 24 to 48 hours in 55% ethanol. Extracts were filtered, concentrated by evaporation under nitrogen, and reconstituted to a known concentration. Extracts were tested for estrogenicity using a yeast reporter gene system expressing human ESR1 and ESR2. Our results suggest that phytoestrogen concentrations vary among plant organs.