Event Title
Vibrio parahemolyticus Bacteriophage: Restriction Enzyme Analysis
Faculty Mentor
Angela Corbin
Location
Orchestra Room, Angelle Hall
Start Date
12-4-2014 10:45 AM
End Date
12-4-2014 11:45 AM
Description
Bacteriophages are host specific viruses that can be used to target bacterial pathogens. The purpose of this experiment was to isolate and purify several Vibrio parahaemolyticus bacteriophages isolated from oyster liquor and open marine waters. Eight phage isolates were tested against 12 different Vibrio sp. to determine host infectivity patterns. Restriction enzyme analysis using BamHI, HaeIII, HambI, ClaI, and EcoRI was performed on the DNA from four isolates. The resulting fragments were separated using electrophoresis. Our results suggest that these bacteriophage have the same fingerprint and therefore may be the same bacteriophage.
Vibrio parahemolyticus Bacteriophage: Restriction Enzyme Analysis
Orchestra Room, Angelle Hall
Bacteriophages are host specific viruses that can be used to target bacterial pathogens. The purpose of this experiment was to isolate and purify several Vibrio parahaemolyticus bacteriophages isolated from oyster liquor and open marine waters. Eight phage isolates were tested against 12 different Vibrio sp. to determine host infectivity patterns. Restriction enzyme analysis using BamHI, HaeIII, HambI, ClaI, and EcoRI was performed on the DNA from four isolates. The resulting fragments were separated using electrophoresis. Our results suggest that these bacteriophage have the same fingerprint and therefore may be the same bacteriophage.