Event Title

Comparative Analysis of A3, A4, and A10 Mycobacteriophages: Sequence Homology at the Repressor Binding Site Results in Homoimmunity of Related Mycobacteriophages

Faculty Mentor

Christopher R. Gissendanner, Allison M.D. Wiedemeier, Ann M. Findley

Location

Orchestra Room, Angelle Hall

Start Date

12-4-2014 10:45 AM

End Date

12-4-2014 11:45 AM

Description

Restriction digestion for cluster assignment was conducted using BamHI, ClaI, EcoRI, HaeIII, and HindIII. A second enzyme panel was identified to distinguish between A2-A10 subcluster members. A lysogen culture of M.smegmatis (prophage Peaches, A4) was utilized to screen isolates for homo/heteroimmunity with Peaches. Rockstar and Trike don’t infect the lysogen culture and were assumed to be A4. The additional restriction panel and sequencing of Rockstar and Trike indicated they were A3 and A10 members, respectively. A repressor binding site sequence homology analysis furnishes evidence for the observed homoimmunity between members of these related, but not identical, cluster representatives.

Comments

Co-Authors: Stephen Jackson, Jobi Arceneaux, Rebecca Baudin, Rudolf Beutner, MaryBeth Borque, Lara Crawford, Amy Fontenot, Brice Gillikin, Mallorie Hayes, Rachel Johnston, Meghan Kurz, Maroutcha Mouawad, Abigayle Reed, Catherine Schilling, Zachary Streeter, John Vu, Tyriana Wilson, Amanda Scott, Meghan Richters, Dustin Lovas, Grant Jernigen, Thai Nguyen, Swapan Bhuiyan, J. Derek Jones, Brittany Miller, Jeremy Harmson

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Apr 12th, 10:45 AM Apr 12th, 11:45 AM

Comparative Analysis of A3, A4, and A10 Mycobacteriophages: Sequence Homology at the Repressor Binding Site Results in Homoimmunity of Related Mycobacteriophages

Orchestra Room, Angelle Hall

Restriction digestion for cluster assignment was conducted using BamHI, ClaI, EcoRI, HaeIII, and HindIII. A second enzyme panel was identified to distinguish between A2-A10 subcluster members. A lysogen culture of M.smegmatis (prophage Peaches, A4) was utilized to screen isolates for homo/heteroimmunity with Peaches. Rockstar and Trike don’t infect the lysogen culture and were assumed to be A4. The additional restriction panel and sequencing of Rockstar and Trike indicated they were A3 and A10 members, respectively. A repressor binding site sequence homology analysis furnishes evidence for the observed homoimmunity between members of these related, but not identical, cluster representatives.