Date of Award
5-2011
Degree Type
Thesis-Restricted
Degree Name
M.S.
Degree Program
Biological Sciences
Department
Biological Sciences
Major Professor
Liu, Zhengchang
Second Advisor
Schluchter, Wendy
Third Advisor
Clancy, Mary J.
Abstract
The CCAAT binding-factor (CBF) is a transcriptional activator conserved in eukaryotes. The CBF in Saccharomyces cerevisiae is a multimeric heteromer termed the Hap2/3/4/5 complex. Hap4, which contains the activation domain of the complex, is also the regulatory subunit and is known to be transcriptionally controlled by carbon sources. However, little is known about Hap4 regulation. In this report, I identify mechanisms by which Hap4 is regulated, including: (1) transcriptional regulation via two short upstream open reading frames (uORFs) in the 5' leader sequence of HAP4 mRNA; (2) proteasome-dependent degradation of Hap4; and (3) identification of two negative regulators of HAP4 expression, CYC8 and SIN4. I also report differential patterns of Hap4 cellular localization which depends on (1) carbon sources, (2) abundance of Hap4 protein, and (3) presence or absence of mitochondrial DNA (mtDNA).
Recommended Citation
Capps, Denise, "The Regulation of NAP4 in Saccharomyces cerevisiae" (2011). University of New Orleans Theses and Dissertations. 116.
https://scholarworks.uno.edu/td/116
Rights
The University of New Orleans and its agents retain the non-exclusive license to archive and make accessible this dissertation or thesis in whole or in part in all forms of media, now or hereafter known. The author retains all other ownership rights to the copyright of the thesis or dissertation