Date of Award
Spring 5-2016
Degree Type
Thesis-Restricted
Degree Name
M.S.
Degree Program
Biological Sciences
Department
Biological Sciences
Major Professor
Dr. Bernard Rees
Second Advisor
Dr. Wendy Schluchter
Third Advisor
Dr. Zhengchang Liu
Abstract
Many fish face low oxygen concentrations (hypoxia) in their natural environments, and they respond to hypoxia through a variety of behavioral, physiological, and cellular mechanisms. Some of these responses involve changes in gene expression. In mammals, the hypoxia inducible factor (HIF) family of transcription factors are the “master regulators” of gene expression during hypoxia, but the study of HIF in fish has been hampered by the lack of reagents to detect this protein in non-mammalian vertebrates. The goals of this thesis are to affinity purify antibodies against HIF from the killifish Fundulus heteroclitus and use them to recover and quantify HIF from killifish cells and tissues. The purified, validated antibodies represent a critical reagent for future studies of the role of HIF in the molecular response of this and other fish to fluctuations in oxygen in their natural environments.
Recommended Citation
Gonzalez-Rosario, Janet, "Purification and characterization of antibodies against killifish HIF-1α" (2016). University of New Orleans Theses and Dissertations. 2150.
https://scholarworks.uno.edu/td/2150
Included in
Biochemistry Commons, Biotechnology Commons, Cellular and Molecular Physiology Commons, Molecular Biology Commons
Rights
The University of New Orleans and its agents retain the non-exclusive license to archive and make accessible this dissertation or thesis in whole or in part in all forms of media, now or hereafter known. The author retains all other ownership rights to the copyright of the thesis or dissertation.