Date of Award
8-2007
Degree Type
Thesis
Degree Name
M.S.
Degree Program
Biological Sciences
Department
Biological Sciences
Major Professor
Clancy, Mary
Second Advisor
Timpte, Candace
Third Advisor
Burt-Utley, Kathleen
Abstract
Developing microbial systems capable of converting low cost lipids into value added products depends on the ability to acquire substrates from the growth media. Saccharomyces cerevisiae can acquire free fatty acids from the growth media and a portion of these lipids can be converted into new lipid products. However, they cannot acquire complex lipids from the growth media unless a nonspecific lipase is included. To circumvent lipase addition, we are genetically engineering S. cerevisiae to secrete a lipase into the growth media. We selected the LIP2 gene from Yarrowia lipolytica, which encodes a nonspecific lipase. Several modifications were made to the LIP2 gene to improve processing. Results identified strains secreting the most lipase. From these results, high producing strains were inserted into an oil inducible vector. Halo assays confirmed lipase secretion, while measuring the fatty acid composition confirmed triacylglycerol breakdown, and yeast uptake of the free fatty acids released.
Recommended Citation
Stewart, Gaynelle, "Engineering Saccharomyces ceresisiae for the Secretion of an Extracellular Lipase" (2007). University of New Orleans Theses and Dissertations. 577.
https://scholarworks.uno.edu/td/577
Rights
The University of New Orleans and its agents retain the non-exclusive license to archive and make accessible this dissertation or thesis in whole or in part in all forms of media, now or hereafter known. The author retains all other ownership rights to the copyright of the thesis or dissertation.