CpeT is the Phycoerythrobilin Lyase for Cys-165 on Beta-Phycoerythrin from Fremyella Diplosiphon and the Chaperone-like Protein CpeZ Greatly Improves its Activity.
Bilin lyases are enzymes which ligate linear tetrapyrrole chromophores to specific cysteine residues on light harvesting proteins present in cyanobacteria and red algae. The lyases responsible for chromophorylating the light harvesting protein phycoerythrin (PE) have not been fully characterized. In this study, we explore the role of CpeT, a putative bilin lyase, in the biosynthesis of PE in the cyanobacterium Fremyella diplosiphon. Recombinant protein studies show that CpeT alone can bind phycoerythrobilin (PEB), but CpeZ, a chaperone-like protein, is needed in order to correctly and efficiently attach PEB to the beta-subunit of PE. MS analyses of the recombinant beta-subunit of PE coexpressed with CpeT and CpeZ show that PEB is attached at Cys-165. Purified phycobilisomes from a cpeT knockout mutant and wild type (WT) samples from F. diplosiphon were analyzed and compared. The cpeT mutant contained much less PE and more phycocyanin than WT cells grown under green light, conditions which should maximize the production of PE. In addition, Northern blot analyses showed that the cpeCDESTR operon mRNAs were upregulated while the cpeBcpeA mRNAs were downregulated in the cpeT mutant strain when compared with WT, suggesting that CpeT may also play a direct or indirect regulatory role in transcription of these operons or their mRNA stability, in addition to its role as a PEB lyase for Cys-165 on beta-PE.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
Nguyen, A. A., Joseph, K. L., Bussell, A. N., Pokhrel, S., Karty, J. A., Kronfel, C. M., Kehoe, D. M., & Schluchter, W. M. (2020). CpeT is the phycoerythrobilin lyase for Cys-165 on beta-phycoerythrin from Fremyella diplosiphon and the chaperone-like protein CpeZ greatly improves its activity. Biochimica et Biophysica Acta, 1861(12). (post print)
This is the accepted version (post-print) of the article. The final published version is available at https://doi.org/10.1016/j.bbabio.2020.148284 .