Date of Award
Bernard B. Rees
The use of viable and reliable tissue preparations allows for experimental approaches in vitro that would not be possible in vivo. In this experiment, gills were isolated from gulf killifish, Fundulus grandis, and subjected to various incubation conditions. A combination of two types of media and two incubation apparatuses were compared in their ability to sustain tissues, as measured by the vital stain trypan blue (TB) and percent lactate dehydrogenase (LDH) leakage. Trypan Blue analysis indicated no significant difference in tissue viability, regardless of the treatment or incubation time. However, percent LDH leakage analysis revealed that the two most influential variables were the medium and apparatus in which the gills were incubated. Interestingly, incubation time did not have a statistically significant influence on gill viability as measured by LDH leakage. The results of LDH leakage analysis suggest that incubation using SW in the 4 ml apparatus is the best combination tested.
Diaz, Joseph Anthony, "In Vitro Incubation of Gills Isolated from the Gulf Killfish, Fundulus Grandis" (2014). Senior Honors Theses. 55.