Date of Award
5-2011
Degree Type
Thesis
Degree Name
M.S.
Degree Program
Biological Sciences
Department
Biological Sciences
Major Professor
Schluchter, Wendy
Second Advisor
Shockey, Jay
Third Advisor
Clancy, Mary J.
Abstract
High-value industrial oils are essential for many processes and have great economic and environmental impacts. The tung tree produces a high-value seed oil. Approximately 80% of tung oil is α-eleostearic acid, which has a high degree of unsaturation thus giving it properties as a drying oil. The identification of the biological components in tung is imperative to further the knowledge of its processes. Four unique tung acyl-CoA binding proteins, VfACBP3a, VfACBP3b, VfACBP4, and VfACBP6 were identified and the genes encoding them were cloned and analyzed to determine their biological roles. The VfACBPs were observed to be similar to other organisms' ACBPs, especially Arabidopsis thaliana. In addition, each gene was expressed in all tung tissues. They were shown to interact with VfDGAT1 and VfDGAT2, two known components of tung lipid metabolism. Finally, VfACBP3a and VfACBP6 were expressed in the seeds of transgenic plants to study the effects of VfACBP expression on seed lipid fatty acid content.
Recommended Citation
Pastor, Steven, "Determining biological roles of four unique Vernicia fordii acyl-CoA Binding Proteins" (2011). University of New Orleans Theses and Dissertations. 1337.
https://scholarworks.uno.edu/td/1337
Rights
The University of New Orleans and its agents retain the non-exclusive license to archive and make accessible this dissertation or thesis in whole or in part in all forms of media, now or hereafter known. The author retains all other ownership rights to the copyright of the thesis or dissertation.