Date of Award
Dr. Zhengchang Liu
Dr. Mary Clancy
Dr. Wendy Schluchter
Hrr25 is a highly conserved serine/threonine protein kinase with diverse functions and it localizes to a number of intracellular sites. Hrr25 possesses an N-terminal kinase domain, a middle region, and a C-terminal proline/glutamine rich domain. In this thesis, we systematically characterized the roles of these three domains of Hrr25 on cell growth, cell morphology, and its cellular localizations. Additionally, we identified a novel target of Hrr25, Pin4. We show that Hrr25-dependent phosphorylation of Pin4 is required for cell wall integrity maintenance. We found that that Hrr25 and Pin4 physically interact in vivo and that the C-terminal domain of Hrr25 is required for this interaction and for maintaining cell wall integrity. Together, we identified a new target and a novel function for Hrr25 and provided insights into the structure-function relationship of Hrr25 domains.
Bhattarai, Amita, "A Novel Role of Hrr25 in Cell Wall Integrity Maintenance and Functional Analysis of Hrr25 Domains" (2019). University of New Orleans Theses and Dissertations. 2663.
Available for download on Monday, August 05, 2024