Date of Award
Dr. Joel Atallah
Dr. Wendy Schluchter
Dr. Zhengchang Liu
Evolutionary developmental biology has historically used generalist model organisms like Drosophila melanogaster. The Hawaiian Drosophilidae radiation, which occurred just 25 million years ago, is an ideal lineage for work in Evo-Devo. Hawaiian flies make up 25% of the world’s Drosophila species and extreme diversity is seen throughout the group. D. grimshawi is a generalist Hawaiian picture-wing fly that has served as a model for the Hawaiian Drosophilidae radiation. However, D. grimshawi’s power and use as a model organism is constrained by our lack of ability to manipulate its genome. In this work, I attempted to edit the genome of D. grimshawi for the first time using a novel CRISPR/Cas9 delivery method known as ReMOT Control. However, my preliminary results may suggest that use of the DmP2C ligand for delivery of the CRISPR/Cas9 components causes injected D. grimshawi females to become transiently sterile, preventing genome editing from occurring with ReMOT Control.
Miller, Bronwyn, "Co-option of the yolkless Oocyte Receptor for CRISPR/Cas9 may Induce Transient Sterility in Female Drosophila grimshawi flies" (2021). University of New Orleans Theses and Dissertations. 2925.