Date of Award

12-2021

Degree Type

Thesis-Restricted

Degree Name

M.S.

Degree Program

Biological Sciences

Department

Biological Sciences

Major Professor

Dr. Joel Atallah

Second Advisor

Dr. Wendy Schluchter

Third Advisor

Dr. Zhengchang Liu

Abstract

Evolutionary developmental biology has historically used generalist model organisms like Drosophila melanogaster. The Hawaiian Drosophilidae radiation, which occurred just 25 million years ago, is an ideal lineage for work in Evo-Devo. Hawaiian flies make up 25% of the world’s Drosophila species and extreme diversity is seen throughout the group. D. grimshawi is a generalist Hawaiian picture-wing fly that has served as a model for the Hawaiian Drosophilidae radiation. However, D. grimshawi’s power and use as a model organism is constrained by our lack of ability to manipulate its genome. In this work, I attempted to edit the genome of D. grimshawi for the first time using a novel CRISPR/Cas9 delivery method known as ReMOT Control. However, my preliminary results may suggest that use of the DmP2C ligand for delivery of the CRISPR/Cas9 components causes injected D. grimshawi females to become transiently sterile, preventing genome editing from occurring with ReMOT Control.

Rights

The University of New Orleans and its agents retain the non-exclusive license to archive and make accessible this dissertation or thesis in whole or in part in all forms of media, now or hereafter known. The author retains all other ownership rights to the copyright of the thesis or dissertation.

Available for download on Saturday, December 17, 2022

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