Date of Award

Fall 12-15-2012

Degree Type

Thesis

Degree Name

M.S.

Degree Program

Biological Sciences

Department

Biological Sciences

Major Professor

Dr. Mary Clancy

Second Advisor

Dr. Wendy Schluchter

Third Advisor

Dr. Zhengchang Liu

Abstract

In Saccharomyces cerevisiae, Ime4 is a protein that is induced during meiosis and has a primary role in regulating sporulation in starving diploids. One function of Ime4 is methylation of adenosine residues within mRNA transcripts. Recent studies have shown Ime4 to be induced in haploids during the mating response, although its role in mating has not been determined. In this report, I identify the subcellular localization of Ime4 during the mating response through treatment with alpha factor. A plasmid containing IME4-GFP under the control of the medium strength promoter CYC1 was created in order to express the protein in a controlled manner. Lastly, mutational analysis was conducted to determine which regions of the protein were necessary for its nuclear localization, aggregation, and sporulation function.

Rights

The University of New Orleans and its agents retain the non-exclusive license to archive and make accessible this dissertation or thesis in whole or in part in all forms of media, now or hereafter known. The author retains all other ownership rights to the copyright of the thesis or dissertation.

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