Date of Award
Fall 12-2012
Degree Type
Thesis
Degree Name
M.S.
Degree Program
Biological Sciences
Department
Biological Sciences
Major Professor
Dr. Mary Clancy
Second Advisor
Dr. Wendy Schluchter
Third Advisor
Dr. Zhengchang Liu
Abstract
In Saccharomyces cerevisiae, Ime4 is a protein that is induced during meiosis and has a primary role in regulating sporulation in starving diploids. One function of Ime4 is methylation of adenosine residues within mRNA transcripts. Recent studies have shown Ime4 to be induced in haploids during the mating response, although its role in mating has not been determined. In this report, I identify the subcellular localization of Ime4 during the mating response through treatment with alpha factor. A plasmid containing IME4-GFP under the control of the medium strength promoter CYC1 was created in order to express the protein in a controlled manner. Lastly, mutational analysis was conducted to determine which regions of the protein were necessary for its nuclear localization, aggregation, and sporulation function.
Recommended Citation
Dehon, Patricia M., "Localization and Mutational Analysis of the Nuclear and Aggregation-Prone Ime4 Protein in Saccharomyces cerevisiae" (2012). University of New Orleans Theses and Dissertations. 1567.
https://scholarworks.uno.edu/td/1567
Rights
The University of New Orleans and its agents retain the non-exclusive license to archive and make accessible this dissertation or thesis in whole or in part in all forms of media, now or hereafter known. The author retains all other ownership rights to the copyright of the thesis or dissertation.