Co-investigators
D.Lovas, M. Richters, S. Bhuyian, J. Harmson, B. Miller
Faculty Mentor
C. R. Gissendanner, A. M. D. Wiedemeier, A. M. Findley
Location
Library 3A
Session
Session 1
Start Date
13-4-2013 9:30 AM
End Date
13-4-2013 10:30 AM
Description
Novel Mycobacteriophages were isolated from soil samples, subjected to repetitive purification plating, and empirical testing that led to the harvesting of high-titer lysates. Lysates were processed for TE M and DNA was isolated from each phage and characterized with selected endonucleases prior to whole genome sequencing. Resultant FAFASTATA files were loaded into the DNA Master and Phamerator bioinformatics workflow to make gene calls and functionally annotate each genome. We present an analysis of homo/heteroimmunity among members of related, but not identical, A subcluster Mycobacteriobacteriophages that is based on lysogen culture infection success, repressor binding site comparisons, and phylogenetic tree-building software.
A Genomic Comparison of Cluster A Mycobacteriophages: Redefining Homoimmunity
Library 3A
Novel Mycobacteriophages were isolated from soil samples, subjected to repetitive purification plating, and empirical testing that led to the harvesting of high-titer lysates. Lysates were processed for TE M and DNA was isolated from each phage and characterized with selected endonucleases prior to whole genome sequencing. Resultant FAFASTATA files were loaded into the DNA Master and Phamerator bioinformatics workflow to make gene calls and functionally annotate each genome. We present an analysis of homo/heteroimmunity among members of related, but not identical, A subcluster Mycobacteriobacteriophages that is based on lysogen culture infection success, repressor binding site comparisons, and phylogenetic tree-building software.