Event Title
Synthesis of Azide Ligands and Conjugation to G5 PAMAM Dendrimers
Faculty Mentor
Stassi DeMaggio
Location
Hamilton Hall 223
Session
Session 2
Start Date
11-4-2014 1:00 PM
End Date
11-4-2014 2:00 PM
Description
Poly(amidoamine) (PAMAM) dendrimers can be used as a platform for drug delivery systems when conjugated to small, functional molecules. However, polydispersity in the conjugated products impedes their translation to the clinic. Due to the large polydispersity of PAMAM dendrimers, a method of isolating monodisperse populations was developed by the conjugation of the dendrimer to polar ligands followed by separation of the monodisperse populations using prep HPLC. The azide ligand, 3-(4-(2-azidoethoxy)phenyl) propanoic acid, was synthesized using a three-step method followed by the conjugation of the purified G5 PAMAM dendrimers through EDC/NHS coupling. High Performance Liquid Chromatography (HPLC) showed distinct resolved populations.
Synthesis of Azide Ligands and Conjugation to G5 PAMAM Dendrimers
Hamilton Hall 223
Poly(amidoamine) (PAMAM) dendrimers can be used as a platform for drug delivery systems when conjugated to small, functional molecules. However, polydispersity in the conjugated products impedes their translation to the clinic. Due to the large polydispersity of PAMAM dendrimers, a method of isolating monodisperse populations was developed by the conjugation of the dendrimer to polar ligands followed by separation of the monodisperse populations using prep HPLC. The azide ligand, 3-(4-(2-azidoethoxy)phenyl) propanoic acid, was synthesized using a three-step method followed by the conjugation of the purified G5 PAMAM dendrimers through EDC/NHS coupling. High Performance Liquid Chromatography (HPLC) showed distinct resolved populations.