Event Title
Phylogeny and diversification of Valerianaceae (Dipsacales).
Faculty Sponsor
Charles Bell
Submission Type
Poster
Description
The Valerianaceae (Dipsacales) is comprised of 250 species distributed worldwide (with the exception of Australia) across a wide ecological gradient. Valerianaceae is a family of the order Dipsacales. They are herbs, less frequently vines, subshrubs or shrubs, sometimes rosette herbs, perennial or annual. A characteristic of this family is the strong odor, especially when dry. Over the past decade, much attention has been paid to the phylogenetic relationships within this clade. Most of these studies, however, have relied exclusively on chloroplast DNA sequence data. For this study, we collected additional data (from both sequences & taxa) to further resolve relationships among the major lineages of Valerianaceae. The phylogenetic utility of each of these several conserved orthologous sequences (COSII markers) was compared to the internal transcribed spacer (ITS) region and several non-coding chloroplast loci that have been widely used in phylogenetic inference of plants. Using already developed primers from a recent study that identified a set of nuclear genes that occur in single copy in multiple sequenced angiosperms, we were able to use sequence data from 4 of these low copy genes. We performed several phylogenetic analyses with these genes and in combination with several chloroplast DNA regions. We used the program PAUP to find the most parsimonious tree that could describe the relations of the different species. Results from these analyses were in strong agreement with previous molecular study and provided additional support for several new hypotheses. / /
Phylogeny and diversification of Valerianaceae (Dipsacales).
The Valerianaceae (Dipsacales) is comprised of 250 species distributed worldwide (with the exception of Australia) across a wide ecological gradient. Valerianaceae is a family of the order Dipsacales. They are herbs, less frequently vines, subshrubs or shrubs, sometimes rosette herbs, perennial or annual. A characteristic of this family is the strong odor, especially when dry. Over the past decade, much attention has been paid to the phylogenetic relationships within this clade. Most of these studies, however, have relied exclusively on chloroplast DNA sequence data. For this study, we collected additional data (from both sequences & taxa) to further resolve relationships among the major lineages of Valerianaceae. The phylogenetic utility of each of these several conserved orthologous sequences (COSII markers) was compared to the internal transcribed spacer (ITS) region and several non-coding chloroplast loci that have been widely used in phylogenetic inference of plants. Using already developed primers from a recent study that identified a set of nuclear genes that occur in single copy in multiple sequenced angiosperms, we were able to use sequence data from 4 of these low copy genes. We performed several phylogenetic analyses with these genes and in combination with several chloroplast DNA regions. We used the program PAUP to find the most parsimonious tree that could describe the relations of the different species. Results from these analyses were in strong agreement with previous molecular study and provided additional support for several new hypotheses. / /