Date of Award
Spring 5-2020
Degree Type
Thesis
Degree Name
M.S.
Degree Program
Biological Sciences
Department
Biological Sciences
Major Professor
Schluchter, Wendy
Second Advisor
Clancy, Mary
Third Advisor
Liu, Zhengchang
Abstract
Marine cyanobacteria of the genus Synechococcushave light harvesting complexes composed of the phycobiliproteins phycoerythrin I and phycoerythrin II. Synechyococcussp RS9916 is capable of altering the ratio of blue-light absorbing phycourobilin to green-light absorbing phycoerythrobilin ligated to these phycoerythrin proteins through a process known as Type IV Chromatic Acclimation (CA4). During CA4, the mpeZ, unk10and fciCgenes are upregulated under blue light, and these three genes are responsible for increasing the phycourobilin content ligated to phycoerythrin I and II. The role of fciCas a transcription activator of mpeZ, andunk10 genes is explored using knockout and overexpression mutants. The role of unk10is explored in phycourobilin ligation to a-phycoerythrin II using a heterologous recombinant protein expression system.
Recommended Citation
Hunter, John M. III, "Characterization of genes involved in Type IV Chromatic Acclimation in Synechococcus RS9916" (2020). University of New Orleans Theses and Dissertations. 2755.
https://scholarworks.uno.edu/td/2755
Rights
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